Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: The proteomic analysis revealed SAA1 as a potential target for pathogenesis in UC. A Significantly expressed proteins (SEPs) were identified between the NC group and DSS group. B A heat map was used to represent the top 20 anomalous proteins in the NC group and DSS group. C Gene Ontology (GO) functional analysis was conducted on the proteins that showed changes in the NC group and DSS group. BP: Biological Process,CC: Cellular Component, MF: Molecular Function. D KEGG pathway enrichment analysis was performed on the proteins that exhibited changes in the NC group and DSS group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Functional Assay

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: Elevated LPS levels and upregulated SAA1/cytokine expression in DSS-induced UC Model. A The expression of LBP in different groups. B and C The levels of proinflammatory cytokine in different groups. (D-F) The protein expression of SAA1, TLR4, p -IKKα/β, IKKβ, p -IκBα, IκBα, p -p65, p65 and β-actin in colon was analyzed by Western blotting and grey value analysis. The results were expressed as mean ± SD, (A-C), n = 10, (D-F), n = 6. * P < 0.05 vs. NC group, ** P < 0.01 vs. NC group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Expressing, Western Blot

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: SAA1 exacerbates the severity of DSS-induced UC model. A Percentage change in body weight from baseline. B Scoring of DAI. C Representative images depicting the colon. D Measurement of colon length. E Representative images showing H&E staining of the colon. F Histological scoring based on the H&E images. G Quantification of colon calprotectin, sIgA, and IgG concentrations. H and I Protein expression analysis by Western blotting and grey value analysis for ZO- 1, occludin, claudin- 1, and β-actin in the colon. The results were expressed as mean ± SD, (A-G), n = 10, (H-I), n = 6. * P < 0.05 vs NC group, ** P < 0.01 vs NC group, # P < 0.05 vs DSS group, ## P < 0.01 vs DSS group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Staining, Expressing, Western Blot

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: SAA1 enhances the expression of proinflammatory cytokines and activates inflammatory signaling pathways. A The expression of LBP was assessed in different groups. B and C The levels of proinflammatory cytokines were measured in different groups. D and E Western blotting and grey value analysis were performed to analyze the protein expression of SAA1, TLR4, p-IKKα/β, IKKβ, p-IκBα, IκBα, p-p65, p65, and β-actin in the colon. F and G Western blot and grey value analysis were conducted to assess the protein expression of NLRP3, pro-Caspase- 1, cleaved-Caspase- 1, ASC IL- 1β, and β-actin in the colon. The results were expressed as mean ± SD, (A-C), n = 10, (D-G), n = 6. * P < 0.05 vs NC group, ** P < 0.01 vs NC group, # P < 0.05 vs DSS group, ## P < 0.01 vs DSS group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Expressing, Protein-Protein interactions, Western Blot

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: SAA1 induced an inflammatory response in intestinal epithelial cells by activating the TLR4/NF-κB signaling pathway and NLRP3 inflammasome. A The effects of different concentrations of LPS on cell proliferation inhibition and IL- 6 concentration were assessed in NCM460 cells after 12 h of exposure. The results are presented as mean ± SD. n = 5. ** p < 0.01 vs 0 μg/ml group. B and C The effect of the SAA1 inhibitor SGA360 on IL- 6 and SAA1 protein expression levels was evaluated in an LPS-induced inflammatory cell model using NCM460 cells. The results are presented as mean ± SD. n = 5. * p < 0.05 vs Control group, ** p < 0.01 vs Control group. D siRNA transfection was used to silence cellular SAA1 expression. The results are presented as mean ± SD. n = 5. * p < 0.05 vs si-Control group, ** p < 0.01 vs si-Control group. E Lentiviral transfection was employed to construct h-SAA1 stable cell lines. The results are presented as mean ± SD. n = 5. ** p < 0.01 vs h-Control group. F The effect of SAA1 on IL- 6 mRNA expression and IL- 6 content in LPS-induced NCM460 cells was investigated. The results are presented as mean ± SD. n = 5. ** p < 0.01 vs Control + LPS group. G Western blotting and gray value analysis were utilized to assess the impact of SAA1 on the expression levels of various proteins including TLR4, p-IKKα/β, IKKβ, p-IκBα, IκBα, p-p65, p65, NLRP3, Caspase- 1, ASC and IL- 1β in LPS-induced NCM460 cells. The results are presented as mean ± SD. n = 5. * p < 0.05 vs Control + LPS group, ** p < 0.01 vs Control + LPS group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Inhibition, Concentration Assay, Expressing, Control, Transfection, Construct, Stable Transfection, Western Blot

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: SAA1 promotes ulcerative colitis by directly activating the TLR4/NF-κB/NLRP3 signaling pathway. The mechanisms by which SAA1 exacerbates the progression of UC involve the activation of the TLR4/NF-κB signaling pathway, promotion of NLRP3 inflammasome activation, induction of TNF-α, IFN-γ, IL- 6 and other pro-inflammatory cytokines, as well as facilitation in inflammatory microenvironment formation. (The diagram was created with FigDraw)

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Activation Assay

Journal: Stroke and Vascular Neurology

Article Title: Extracellular vesicles bearing serum amyloid A1 exacerbate neuroinflammation after intracerebral haemorrhage

doi: 10.1136/svn-2024-003525

Figure Lengend Snippet: Human serum amyloid A1 (SAA1) protein levels in plasma and extracellular vesicles correlated (EVs) with clinical and laboratory assessments. ( A, B ) Bar graph indicating the concentration of SAA1 protein in both EVs and plasma for the ICH group and the healthy controls group. Unit: ng/mL in EVs and μg/mL in plasma. **p<0.01, ***p<0.001 by the two-tailed unpaired Student’s t-test and data are presented as means±SEM. ( A ) n=6, 15 in the control and ICH group, ( B ) n=13, 19 in the control and the ICH group. ( C, D ) Linear dependence graph revealing the relationship between neutrophils or leucocytes and the SAA1 protein in EVs (n=15). Correlation was analysed by spearman correlation analysis and the dashed line indicates the 95% CI. ( E–H ) Correlation analysis between the EVs-derived, plasma-derived SAA1 levels and the clinical assessments, including NIHSS and haemorrhage volume, through a linear dependence graph (n=15). Correlation was analysed by spearman correlation analysis and the dashed line indicates the 95% CI. ICH, intracerebral haemorrhage; NIHSS, National Institutes of Health Stroke Scale.

Article Snippet: To evaluate the exogenous effect of SAA1 on the mouse brain, we administered varying doses (0 ng, 83 ng, 250 ng, 750 ng) of SAA1 protein (CSB-EP020656MO, CUSABIO, China) dissolved in 1 μL of saline via intracerebral injection.

Techniques: Clinical Proteomics, Concentration Assay, Two Tailed Test, Control, Derivative Assay

Journal: Stroke and Vascular Neurology

Article Title: Extracellular vesicles bearing serum amyloid A1 exacerbate neuroinflammation after intracerebral haemorrhage

doi: 10.1136/svn-2024-003525

Figure Lengend Snippet: Exogenous SAA1 upregulates the cell counts of microglia and astrocytes. ( A ) Schematic representation of exogenous SAA1 inserting into the basal ganglia region at varying dosages (750 ng, 250 ng, 83 ng and 0 ng). Images of immunostaining of microglia and astrocyte activation on day three are provided. ( B, D ) Images of immunostaining ( B ) and quantification ( D ) of microglia (stained by a specific marker, Iba1) around the ipsilateral basal ganglia region at different doses of exogenous SAA1. Microglia counts per mm 2 : 9.3±2.2, 41.5±3.9, 64.0±4.0, 88.3±8.8 for 0 ng, 83 ng, 250 ng, and 750 ng, respectively. ( C, E ) Images of immunostaining of GFAP (glial fibrillar acidic protein, known as the astrocyte active marker) representing astrocytes ( C ) and quantification of astrocytes ( E ) at different doses of exogenous SAA1 in the ipsilateral basal ganglia region. Astrocyte counts per mm 2 : 36.0±3.8, 93.5±6.1, 134.0±3.5, 176.5±11.6 for 0 ng, 83 ng, 250 ng, and 750 ng, respectively. Scale bars=100 µm, inset scale bars=20 µm, n=4 in each group. ***p<0.001 by the Kruskal-Wallis test. Data are presented as means±SEM.

Article Snippet: To evaluate the exogenous effect of SAA1 on the mouse brain, we administered varying doses (0 ng, 83 ng, 250 ng, 750 ng) of SAA1 protein (CSB-EP020656MO, CUSABIO, China) dissolved in 1 μL of saline via intracerebral injection.

Techniques: Immunostaining, Activation Assay, Staining, Marker

Journal: Stroke and Vascular Neurology

Article Title: Extracellular vesicles bearing serum amyloid A1 exacerbate neuroinflammation after intracerebral haemorrhage

doi: 10.1136/svn-2024-003525

Figure Lengend Snippet: Blocking SAA1 promotes microglia reactivity and leucocyte infiltration. ( A ) Bar graph indicating the elevation of plasma SAA1 levels in ICH mice as compared with the sham group. n= 5 mice in the ICH group and n=10 mice in the sham group. **p<0.01 by two-tailed unpaired Student’s t-test. ( B ) Schematic diagram depicting intracerebral haemorrhage induction followed by intravenous administration of anti-SAA1 mAb or IgG 1 hour later. Immune cell populations were assessed using flow cytometry on days 1 and 3 post-ICH initiation. ( C ) Flow cytometry gating strategy depicting immune cell populations in mice brain treated with anti-SAA1 antibody or IgG on days 1 and day three post-ICH induction. The graph illustrates CD45 high leucocytes, including CD3 + CD19 - T lymphocytes and its subtypes: CD4 + CD8 − T and CD4 - CD8 + T lymphocytes, CD3 − CD19 + B lymphocytes, CD11b + Ly6G + neutrophils, and CD11b + F4/80 + macrophages. It also illustrates CD45 int CD11b + microglia, including its subtypes: CD86 + microglia and CD206 + microglia. All gates were set using fluorescence-minus-one (FMO) controls. ( D ) Bar graph indicates the number of microglia and their subtypes in ICH mice with anti-SAA1 antibody or IgG treatment from days 1 to 3. ( E ) Bar graph shows major brain infiltrated leucocytes, involving CD8 + T lymphocytes, B lymphocytes, and neutrophils in ICH mice with anti-SAA1 antibody or IgG treatment from days 1 to 3. Int, intermediate. n=5, 6, 5 on day 1 and n=5, 13, 10 on day 3 for sham, IgG and mAb group. n=5, 9, 7 on day 3 for CD86 + microglia group in sham, IgG and mAb group specially. *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA and Tukey’s test. Data are presented as means±SEM. ANOVA, analysis of variance; ICH, intracerebral haemorrhage.

Article Snippet: To evaluate the exogenous effect of SAA1 on the mouse brain, we administered varying doses (0 ng, 83 ng, 250 ng, 750 ng) of SAA1 protein (CSB-EP020656MO, CUSABIO, China) dissolved in 1 μL of saline via intracerebral injection.

Techniques: Blocking Assay, Clinical Proteomics, Two Tailed Test, Flow Cytometry, Fluorescence

Journal: Stroke and Vascular Neurology

Article Title: Extracellular vesicles bearing serum amyloid A1 exacerbate neuroinflammation after intracerebral haemorrhage

doi: 10.1136/svn-2024-003525

Figure Lengend Snippet: Anti-SAA1 mAb administration alleviates brain injury in mice with ICH. ( A ) Schematic diagram illustrating the ICH followed by intravenous injection of anti-SAA1 mAb or IgG 1 hour later. Subsequently, mice underwent MRI and neurological evaluations on days 1 and 3 after ICH induction. ( B ) Neurological scores of the sham group and the ICH group treated with anti-SAA1 mAb or IgG on day 1 and day 3. The modified Neurological Severity Score (mNSS) and rotarod test were used to measure the neurological deficit. n=6, 12, 7 for sham, IgG and mAb group. *p<0.05 by two-way ANOVA. ( C ) MRI of lesion volume (red) and perihaemorrhagic oedema (PHE) volume (yellow) on day 1 and day 3 post-ICH. ( D, E ) Quantification of lesion volume and PHE volume using the MRI. n=4, 6 for IgG and mAb group on day 1, n=5, 8 for IgG and mAb group on day 3. *p<0.05 by two-tailed unpaired Student’s t-test. Data are presented as means±SEM. ANOVA, analysis of variance; ICH, intracerebral haemorrhage.

Article Snippet: To evaluate the exogenous effect of SAA1 on the mouse brain, we administered varying doses (0 ng, 83 ng, 250 ng, 750 ng) of SAA1 protein (CSB-EP020656MO, CUSABIO, China) dissolved in 1 μL of saline via intracerebral injection.

Techniques: Injection, Modification, Two Tailed Test

Journal: bioRxiv

Article Title: Modulation of metabolic, inflammatory, fibrotic and cell death pathways by Resmetirom in metabolic dysfunction-associated steatohepatitis (MASH): A transcriptomic profiling study

doi: 10.1101/2025.11.26.690715

Figure Lengend Snippet: (A) Expression of thyroid hormone receptor-β ( THR-β ) and thyroid hormone receptor-α ( THR-α ) in human liver tissue across MASLD progression stages (GSE213621). (B) Experimental design: male C57BL/6J mice were fed a fructose, palmitate, and cholesterol (FPC) diet for 12 weeks. Therapeutic Resmetirom (Rem) (5 mg/kg/day) or vehicle (0.5% carboxymethyl cellulose-sodium + 0.4% Tween-80) was administered intraperitoneally from week 4 to week 12. (C) Body weight changes over the 12-week period. (D) Representative images and quantification of hepatic lipid accumulation by Nile Red, BODIPY, and hematoxylin & eosin (H&E) staining. Scale bar: 50 μm for Nile Red and BODIPY; Scale bar: 100 μm for H&E. (E–F) Serum biochemical analyses, including total cholesterol (TC), triglycerides (TG), high-density-lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). (G) Total cholesterol and triglyceride content in liver. (H) Immunofluorescence analysis of macrophage infiltration (CD68). Scale bar: 50 μm. (I-J) Serum levels of systemic inflammation markers (CRP and SAA1). (K–L) Sirius Red and immunofluorescence staining of α-smooth muscle actin (α-SMA) indicating fibrosis. Scale bar: 100 μm for Sirius Red; Scale bar: 50 μm for α-SMA. (M) TUNEL assay for hepatocyte apoptosis. Scale bar: 100 μm. (N) Principal component analysis (PCA) of hepatic transcriptome showing distinct clustering between vehicle- and Resmetirom-treated groups (n=4). (O) Volcano plot depicting differentially expressed genes (DEGs) between groups (p < 0.05, |log 2 FC| > 0.5). (P) Pathway enrichment analysis of DEGs highlighting key biological processes. (Q) Heatmap of representative DEGs associated with hepatic steatosis, inflammation, fibrosis, and cell death. Statistical significance: p < 0.05.

Article Snippet: To assess systemic inflammatory markers, serum concentrations of C-reactive protein (CRP; #KE10128, Proteintech) and serum amyloid A1 (SAA1; #CSB-EL020656MO, CUSABIO) were determined using specific ELISA kits following the manufacturers’ instructions.

Techniques: Expressing, Staining, Immunofluorescence, TUNEL Assay

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Primer sequences used for quantitative PCR

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Radiation time response in mouse SAA1 measured using ELISA in 8 Gy irradiated female C57BL/6J mice at 0, 1, 2, 4, and 6 hours post-irradiation. n=3 per group. *, P<0.05 in the irradiated mice compared with control mice.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Time and dose response of SAA1 after TBI. (A) SAA1 was measured using ELISA in 0, 1, 2, 4, 8 and 12 Gy irradiated female C57BL/6J mice at 0.125, 0.5, 1, 2, 3, 5 and 7 days post-irradiation. SAA1 dose-dependent change at (B) 0.25, (C) 0.5, (D) 1, (E) 2, (F) 3, (G) 5 and (H) 7 days after exposure to radiation. Each black dot represents one animal. Error bars indicate ± 1 SD for each radiation exposure group. n=8 per group (n=7 at 7 days after 8 Gy, n=2 at 7 days after 12 Gy). SAA1, serum amyloid A1; TBI, total body irradiation.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: P values associated with two-tailed Student’s t -test for SAA1 concentrations after comparing control and irradiated groups

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 mRNA time-dependent increase after radiation in (A) liver, (B) lung, (C) thymus, (D) spleen, (E) bone marrow and (F) small intestine measured using Quantitative PCR in control and 8 Gy irradiated female C57BL/6J mice at 0.125, 0.5, 1, 2, 3, 5 and 7 days post-irradiation. Error bars indicate ± 1 SD for each radiation exposure group. n=6 to 8 per group. *, P<0.05, *, P<0.01, ***, P<0.001, and ****, P<0.0001 in the irradiated mice compared with the control mice. SAA1, serum amyloid A1.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Real-time Polymerase Chain Reaction, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 expression and systemic infection parameters in the same mice. (A) SAA1 and LPS in serum and 16S rRNA in the liver of the same animals measured on 31 mice on day 0,1, 5 and 7 (7 mice on day 7, and 8 per group on day 0, 1 and 5) after exposure to 8 Gy TBI. (B) SAA1 and PCT in serum in the same animals was on 23 mice on days 0, 0.125, 0.5, 1, 2, 3, 5 and 7 (2 mice on days 7 and 3 per group on days 0, 0.125, 0.5, 1, 2, 3 and 5) after exposure to 12 Gy TBI. SAA1, serum amyloid A1; TBI, total body irradiation.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Expressing, Infection, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Multiple linear regression analysis between serum SAA1 concentration and number of lymphocyte and irradiation dose

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 cut-off values of classification within 2 days post-irradiation

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: PBI patterns and SAA1 response. (A) The liver of C57BL/6J mice was shielded in PBI-1 and PBI-3 and in the corresponding groups PBI-2 and PBI-4 the liver was exposed. (B) SAA1 concentration in serum and (C) mRNA expression in liver of control, total-body irradiation and PBI group at 12 hours after 8 Gy irradiation. Error bars indicate ±1 SD for each radiation exposure group. n=6 per group. **, P<0.01, ***, P<0.001, and ****, P<0.0001 in the irradiated mice compared with the control mice. PBI, partial body irradiation; SAA1, serum amyloid A1.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Expressing, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Dynamic SAA1 concentration and corresponding date of death within 30 days after 10 Gy irradiation

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Mean and standard deviation (SD) of SAA1 concentration in 92 healthy mice

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Standard Deviation, Concentration Assay

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Kaplan-Meier survival curves of mice. P value determined by log-rank test. Serum SAA1 in mice treated with amifostine before 10 Gy irradiation and in the 10 Gy group on day −4, 1, 3, 5 and 7. n=8 in 10 Gy + Amifostine group, n=20 in 10 Gy group. ***, P<0.001. SAA1, serum amyloid A1.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 concentration in the Amifostine group and corresponding death date within 30 days

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Clinical parameters and corresponding SAA1 concentration in all 17 NPC patients

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Effect of radiotherapy on serum SAA1 in nasopharyngeal carcinoma patients. (A) The scatter plot shows SAA1 concentration before radiotherapy and corresponding expression after radiotherapy in 17 patients with nasopharyngeal carcinoma (****, P<0.0001). (B) ROC curve of SAA1 as a biomarker for predicting radiation exposure in patients with nasopharyngeal carcinoma. SAA1, serum amyloid A1.

Article Snippet: ELISA measurement Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Expressing, Biomarker Assay